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rabbit anti ezh2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti ezh2
    Rabbit Anti Ezh2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+ezh2+antibody/Ezh2+XP+Rabbit+mAb/pm41888115-297-35-41
    Average 96 stars, based on 1127 article reviews
    rabbit anti ezh2 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Staining:

    Article Title: EZH2 inhibition or genetic ablation suppresses cyst growth in autosomal dominant polycystic kidney disease.
    Article Snippet: .. For EZH2 staining, a rabbit anti-EZH2 antibody (#5246S, 1:50, CST) was used. .. Ki-67 was stained with anti-Ki-67 (#ab15580, 1:100; Abcam).

    Article Title: EZH2 inhibition or genetic ablation suppresses cyst growth in autosomal dominant polycystic kidney disease
    Article Snippet: .. For EZH2 staining, a rabbit anti-EZH2 antibody (#5246S, 1:50, CST) was used. .. Ki-67 was stained with anti-Ki-67 (#ab15580, 1:100; Abcam).

    Incubation:

    Article Title: PHF19 drives PRC2 sub-nuclear compartmentalization to promote motility in TNBC cells
    Article Snippet: .. The cells were then incubated with the rabbit anti-EZH2 antibody (5246, Cell signaling, USA) for 4 hours at RT, washed 3 times with PBST for 5 min and then incubated with Alexa FluorTM 647 secondary antibody (A-21245, ThermoFisher, USA) for 2 hours. ..

    Article Title: PHF19 drives the formation of PRC2 clusters to enhance motility in TNBC cells.
    Article Snippet: .. The cells were then incubated with the rabbit anti-EZH2 antibody (5246, Cell signaling, USA) for 4 h at RT, washed 3 times with PBST for 5 min and then incubated with Alexa Fluor 647 secondary antibody (A-21245, ThermoFisher, USA) for 2 h. The image processing function in Microscoop was used to generate a mask for EZH2 clusters, which automatically guided the photolabeling process within the masked regions across the entire cell chamber slide. .. Upon completion of the photolabeling process, the cells were quenched with the quencher in Synlight-RichTM kit (SYN-RI0106, Syncell, Taiwan) for 3 times wash, each for 5 min.

    Article Title: Targeting IL-11R/EZH2 signaling axis as a therapeutic strategy for osteosarcoma lung metastases
    Article Snippet: Antigen retrieval via heating with EDTA (pH 8.0; Zymed Laboratories) was followed by biotin and protein blocking (Dako). .. Expression of EZH2 was evaluated using a rabbit anti-EZH2 antibody (clone AC22; Cell Signaling Technology) diluted at 1:15 (vol/vol) and incubated for 45 min, followed by development using an LSAB + Kit (Dako). ..

    Article Title: PHF19 drives the formation of PRC2 clusters to enhance motility in TNBC cells.
    Article Snippet: The immunoprecipitation was performed in IP buffer, which was also used as wash buffer and consisted of 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 2 mM MgCl2, 0.5% NP-40, 10% glycerol, protease inhibitors (Roche) and phosphatase inhibitors. .. Equal amounts of nuclear lysates (0.4–0.8 mg per IP reaction as quantified by a Bradford assay), were mixed with 1 μg of rabbit anti-EZH2 antibody (5246, Cell signaling, USA) or 1 μg of rabbit (DA1E) mAb IgG XP Isotype Control (3900S, Cell Signaling USA) and incubated end-over-end for 16 h at 4 ◦ C. The immunocomplexes were retrieved by incubation with 30 μL protein Protein G Agarose beads (LGC SeraCare – KPL, 5720-0001) for 2 h at 4 ◦ C. The beads were subsequently washed five times with IP buffer before elution in 4× Bolt LDS Sample buffer (ThermoFischer Scientific, B0008) with 50 nM DTT, at 70 ◦ C for 10 min. .. For transient knockdowns, cells were reverse transfected using Lipofectamine RNAiMAX (Invitrogen) at a final concentration of 5nM total siRNA, according to manufacturer’s instructions.

    Negative Control:

    Article Title: Epigenetically silencing gluconeogenic enzyme PCK1 by EZH2 promotes renal tubulointerstitial fibrosis
    Article Snippet: CUT&Tag experiments were performed following the procedures described in the CUT&Tag-seq section by using Hyperactive In-Situ ChIP Library Prep Kit for Illumina kit (Vazyme Biotech, TD901). .. Rabbit anti-EZH2 antibody (CST, 5246), the negative control rabbit IgG (CST, 3900) and the positive control Pol II antibody (CST, 2629) were used. ..

    Positive Control:

    Article Title: Epigenetically silencing gluconeogenic enzyme PCK1 by EZH2 promotes renal tubulointerstitial fibrosis
    Article Snippet: CUT&Tag experiments were performed following the procedures described in the CUT&Tag-seq section by using Hyperactive In-Situ ChIP Library Prep Kit for Illumina kit (Vazyme Biotech, TD901). .. Rabbit anti-EZH2 antibody (CST, 5246), the negative control rabbit IgG (CST, 3900) and the positive control Pol II antibody (CST, 2629) were used. ..

    Expressing:

    Article Title: Targeting IL-11R/EZH2 signaling axis as a therapeutic strategy for osteosarcoma lung metastases
    Article Snippet: Antigen retrieval via heating with EDTA (pH 8.0; Zymed Laboratories) was followed by biotin and protein blocking (Dako). .. Expression of EZH2 was evaluated using a rabbit anti-EZH2 antibody (clone AC22; Cell Signaling Technology) diluted at 1:15 (vol/vol) and incubated for 45 min, followed by development using an LSAB + Kit (Dako). ..

    Bradford Assay:

    Article Title: PHF19 drives the formation of PRC2 clusters to enhance motility in TNBC cells.
    Article Snippet: The immunoprecipitation was performed in IP buffer, which was also used as wash buffer and consisted of 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 2 mM MgCl2, 0.5% NP-40, 10% glycerol, protease inhibitors (Roche) and phosphatase inhibitors. .. Equal amounts of nuclear lysates (0.4–0.8 mg per IP reaction as quantified by a Bradford assay), were mixed with 1 μg of rabbit anti-EZH2 antibody (5246, Cell signaling, USA) or 1 μg of rabbit (DA1E) mAb IgG XP Isotype Control (3900S, Cell Signaling USA) and incubated end-over-end for 16 h at 4 ◦ C. The immunocomplexes were retrieved by incubation with 30 μL protein Protein G Agarose beads (LGC SeraCare – KPL, 5720-0001) for 2 h at 4 ◦ C. The beads were subsequently washed five times with IP buffer before elution in 4× Bolt LDS Sample buffer (ThermoFischer Scientific, B0008) with 50 nM DTT, at 70 ◦ C for 10 min. .. For transient knockdowns, cells were reverse transfected using Lipofectamine RNAiMAX (Invitrogen) at a final concentration of 5nM total siRNA, according to manufacturer’s instructions.

    Control:

    Article Title: PHF19 drives the formation of PRC2 clusters to enhance motility in TNBC cells.
    Article Snippet: The immunoprecipitation was performed in IP buffer, which was also used as wash buffer and consisted of 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 2 mM MgCl2, 0.5% NP-40, 10% glycerol, protease inhibitors (Roche) and phosphatase inhibitors. .. Equal amounts of nuclear lysates (0.4–0.8 mg per IP reaction as quantified by a Bradford assay), were mixed with 1 μg of rabbit anti-EZH2 antibody (5246, Cell signaling, USA) or 1 μg of rabbit (DA1E) mAb IgG XP Isotype Control (3900S, Cell Signaling USA) and incubated end-over-end for 16 h at 4 ◦ C. The immunocomplexes were retrieved by incubation with 30 μL protein Protein G Agarose beads (LGC SeraCare – KPL, 5720-0001) for 2 h at 4 ◦ C. The beads were subsequently washed five times with IP buffer before elution in 4× Bolt LDS Sample buffer (ThermoFischer Scientific, B0008) with 50 nM DTT, at 70 ◦ C for 10 min. .. For transient knockdowns, cells were reverse transfected using Lipofectamine RNAiMAX (Invitrogen) at a final concentration of 5nM total siRNA, according to manufacturer’s instructions.



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    Cell Signaling Technology Inc ezh2
    Anti-transcription activity of γPNA1 with HDACi in lymphoma cells Relative fold change of c-Myc levels in U2932 cells measured by real-time PCR on day 2 after treatment with (A) γPNA1 and (B) ScR-γPNA2 in combination with romidepsin, entinostat, vorinostat, panobinostat, and belinostat. Results are presented as mean ± SEM and two-way ANOVA was used to determine the statistically significant difference between groups. Western blot analysis representing the change in c-MYC protein on day 2 after treatment with γPNA1 and ScR-γPNA2 in combination with (C) romidepsin, (D) entinostat, (E) vorinostat, (F) panobinostat, and (G) belinostat. ∗∗(C–F) Cyclophilin B was used as an endogenous control, and the same blots are presented in C–S3G. c-MYC, <t>EZH2,</t> and cyclophilin B were probed from the same blot. Results are presented as mean ± SEM, and the p value between groups was determined using one-way ANOVA.
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    Cell Signaling Technology Inc anti ezh2
    Anti-transcription activity of γPNA1 with HDACi in lymphoma cells Relative fold change of c-Myc levels in U2932 cells measured by real-time PCR on day 2 after treatment with (A) γPNA1 and (B) ScR-γPNA2 in combination with romidepsin, entinostat, vorinostat, panobinostat, and belinostat. Results are presented as mean ± SEM and two-way ANOVA was used to determine the statistically significant difference between groups. Western blot analysis representing the change in c-MYC protein on day 2 after treatment with γPNA1 and ScR-γPNA2 in combination with (C) romidepsin, (D) entinostat, (E) vorinostat, (F) panobinostat, and (G) belinostat. ∗∗(C–F) Cyclophilin B was used as an endogenous control, and the same blots are presented in C–S3G. c-MYC, <t>EZH2,</t> and cyclophilin B were probed from the same blot. Results are presented as mean ± SEM, and the p value between groups was determined using one-way ANOVA.
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    Image Search Results


    Anti-transcription activity of γPNA1 with HDACi in lymphoma cells Relative fold change of c-Myc levels in U2932 cells measured by real-time PCR on day 2 after treatment with (A) γPNA1 and (B) ScR-γPNA2 in combination with romidepsin, entinostat, vorinostat, panobinostat, and belinostat. Results are presented as mean ± SEM and two-way ANOVA was used to determine the statistically significant difference between groups. Western blot analysis representing the change in c-MYC protein on day 2 after treatment with γPNA1 and ScR-γPNA2 in combination with (C) romidepsin, (D) entinostat, (E) vorinostat, (F) panobinostat, and (G) belinostat. ∗∗(C–F) Cyclophilin B was used as an endogenous control, and the same blots are presented in C–S3G. c-MYC, EZH2, and cyclophilin B were probed from the same blot. Results are presented as mean ± SEM, and the p value between groups was determined using one-way ANOVA.

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: Combining anti-gene γPNA with small molecules and RNA inhibitors: A strategy to enhance anti-tumor efficacy

    doi: 10.1016/j.omtn.2025.102804

    Figure Lengend Snippet: Anti-transcription activity of γPNA1 with HDACi in lymphoma cells Relative fold change of c-Myc levels in U2932 cells measured by real-time PCR on day 2 after treatment with (A) γPNA1 and (B) ScR-γPNA2 in combination with romidepsin, entinostat, vorinostat, panobinostat, and belinostat. Results are presented as mean ± SEM and two-way ANOVA was used to determine the statistically significant difference between groups. Western blot analysis representing the change in c-MYC protein on day 2 after treatment with γPNA1 and ScR-γPNA2 in combination with (C) romidepsin, (D) entinostat, (E) vorinostat, (F) panobinostat, and (G) belinostat. ∗∗(C–F) Cyclophilin B was used as an endogenous control, and the same blots are presented in C–S3G. c-MYC, EZH2, and cyclophilin B were probed from the same blot. Results are presented as mean ± SEM, and the p value between groups was determined using one-way ANOVA.

    Article Snippet: Primary antibodies for c-MYC (Cell Signaling Technology, #5605) and EZH2 (Cell Signaling Technology, #5246) were applied using a rabbit monoclonal antibody diluted in 3% BSA at 4°C overnight.

    Techniques: Activity Assay, Real-time Polymerase Chain Reaction, Western Blot, Control

    MYC/MAX inhibitors in combination with anti-transcription γPNA1 Cell viability of (A) U2932 and (B) Raji cells treated with increasing doses of MYC/MAX inhibitors (Myci975, EN4, 10058-F4, and sAJM589) alone and in combination with γPNA1 and ScR-γPNA2 (8 μM) for 72 h. Results are presented as mean ± SEM. The IC 50 (95% CI) values of MYC/MAX inhibitors alone and combination treatment of MYC/MAX with γPNA1 in (C) U2932 and (D) Raji cells. (E) Cell viability of γPNA1-treated U2932 and Raji cells at 8 μM concentration. Western blot analysis representing the change in c-MYC protein 72 h after treatment with γPNA1 and ScR-γPNA2 in combination with (F) Myci975, (G) EN4, (H) 10058-F4, and (I) sAJM589. ∗∗(F–I) Cyclophilin B was used as an endogenous control, and the same blots are presented in A–S7D. c-MYC, EZH2, and cyclophilin B were probed from the same blot. Results are presented as mean ± SEM, p value for one-way ANOVA.

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: Combining anti-gene γPNA with small molecules and RNA inhibitors: A strategy to enhance anti-tumor efficacy

    doi: 10.1016/j.omtn.2025.102804

    Figure Lengend Snippet: MYC/MAX inhibitors in combination with anti-transcription γPNA1 Cell viability of (A) U2932 and (B) Raji cells treated with increasing doses of MYC/MAX inhibitors (Myci975, EN4, 10058-F4, and sAJM589) alone and in combination with γPNA1 and ScR-γPNA2 (8 μM) for 72 h. Results are presented as mean ± SEM. The IC 50 (95% CI) values of MYC/MAX inhibitors alone and combination treatment of MYC/MAX with γPNA1 in (C) U2932 and (D) Raji cells. (E) Cell viability of γPNA1-treated U2932 and Raji cells at 8 μM concentration. Western blot analysis representing the change in c-MYC protein 72 h after treatment with γPNA1 and ScR-γPNA2 in combination with (F) Myci975, (G) EN4, (H) 10058-F4, and (I) sAJM589. ∗∗(F–I) Cyclophilin B was used as an endogenous control, and the same blots are presented in A–S7D. c-MYC, EZH2, and cyclophilin B were probed from the same blot. Results are presented as mean ± SEM, p value for one-way ANOVA.

    Article Snippet: Primary antibodies for c-MYC (Cell Signaling Technology, #5605) and EZH2 (Cell Signaling Technology, #5246) were applied using a rabbit monoclonal antibody diluted in 3% BSA at 4°C overnight.

    Techniques: Concentration Assay, Western Blot, Control